This web page was produced as an assignment for Genetics 677, an undergraduate course at UW-Madison.
Multiple alignments were performed using Clustal W, T-Coffee, MUSCLE and GeneBee programs. These were then used to create phylogenetic trees representing possible evolutionary relationships. Each phylogenetic tree was determined using slightly different sequence alignments, yet each resulted in very similar results.
COL1A1 Phylogenetic Tree
Figure 1. Phylogenetic tree created based upon multiple alignments performed by Clustal W 2.0.3 software.
Figure 2. Phylogenetic tree created based upon multiple alignments obtained from the use of MUSCLE 3.7 software. It is worthwhile to note this tree's similarity to the previous one.
Figure 3. Phylogenetic tree created using multiple alignments produced by T-COFFEE 6.85 software.
Figure 4. Phylogenetic tree proposed by GeneBee mulitple alignment software. Notice the slight difference between this tree and the previous three in terms of the evolutionary relationship of the COL1A1 protein.
Below is a downloadable text file of all FASTA protein sequences used when performing the multiple alignments used to construct the above phylogenetic trees.
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